Project Description

Diana Gudynaite

Diana Gudynaite

Early Stage Researcher 12b,
University of Dundee

Project Title and Work Package(s): Genetic study of the molecular mechanisms of sensory perception in B. subtilis. (WP 4)

Objectives: To: (1) generate σB reporter fusions that allow the quantification of stressosome-dependent stress sensing in B subtilis; (2) Use random transposon mutagenesis and targeted mutagenesis of RsbR and its paralogues to identify mutants with defective stress sensing (either locked-on or blind); (3) develop single-cell and population based mathematical models that account for the stress sensing behaviour of B. subtilis in collaboration with participant #UC.

Expected Results: The development of stress-sensing biosensors for B. subtilis, which will be available to the Network. Identification of novel factors influencing stressosome-mediated sensing in B. subtilis and a genetic definition of residues important for sensing in RsbR and its paralogues. A model describing the molecular mechanisms of stress sensing in Bacillus.

Personal Statement

I am excited to join the PATHSENSE project at the University of Dundee, Scotland. Recently I have completed a Master’s degree in Biotechnology at the National University of Ireland Galway, and previously a Bachelor’s degree in Nutraceutical science (TU Dublin). During the MSc research project explored the causes of acid tolerance in Listeria monocytogenes. During that project, I began to develop a strong interest in the pathogen’s stress coping mechanisms and how they get activated. For my PATHSENSE project the aim is to gain a better understanding of the stressosome signalling pathway in Bacillus subtilis, via the RsbR protein molecule and its paralogues