Project Title and Work Package(s): Genetic study of the molecular mechanisms of sensory perception in B. subtilis. (WP 4)
Objectives: To: (1) generate σB reporter fusions that allow the quantification of stressosome-dependent stress sensing in B subtilis; (2) Use random transposon mutagenesis and targeted mutagenesis of RsbR and its paralogues to identify mutants with defective stress sensing (either locked-on or blind); (3) develop single-cell and population based mathematical models that account for the stress sensing behaviour of B. subtilis in collaboration with participant #UC.
Expected Results: The development of stress-sensing biosensors for B. subtilis, which will be available to the Network. Identification of novel factors influencing stressosome-mediated sensing in B. subtilis and a genetic definition of residues important for sensing in RsbR and its paralogues. A model describing the molecular mechanisms of stress sensing in Bacillus.