In our group we use global as well as gene specific approaches to investigate the pathophysiology of Staphylococcus aureus.
We are particularly interested in the global metabolic adaptation of S. aureus to different carbon sources and how the carbon source modulates virulence gene expression. In addition, to providing a global and quantitative view of the S. aureus physiology, these studies always identify a large number of proteins of unknown function. Therefore, in addition to the bird´s eye view provide by proteomics, we are also interested in the characterization of these proteins to uncover their molecular function in stress adaptation. During the last years it became evident that localization matters even in the seemingly simple world of bacterial cells. Consequently, a focus is put on the analysis of the spatial distribution of these proteins and how localization relates to function.
Furthermore, we are also interested in the evolution and function of signaling pathways controlled by the stressosome, a large 1.8 MDa cytosolic protein complex that was first described in the soil bacterium Bacillus subtilis. Using proteomics and phenotypic assays we map the biological processes controlled by the stressosome in Gram-negative bacteria including animal and plant pathogens to unravel the functional and regulatory diversity of the stressosome pathway.
To achieve these goals and since proteomics shows only one side of the coin, we enjoy collaborations with many other groups from the University of Greifswald and across Europe.