Project Title and Work Package(s): Genetic characterisation of the L. monocytogenes stressosome and role in virulence and food survival (WP2, WP3)
Objectives: To: (1) construct defined mutants of L. monocytogenes lacking stressosome components and characterise their phenotypes in relation to stress tolerance and virulence; (2) with UmU identify novel regulatory components that interact with the stressosome using transposon mutagenesis; (3) test the role of the stressosome in virulence and in survival in food matrices.
Expected Results: A detailed understanding of the contribution of each stressosome sensory protein (RsbR and paralogues) to stress sensing and stress response.
Identification of regulatory components that interact with the stressosome. A model describing the role of the L. monocytogenes stressosome in virulence and survival in the food chain.