Project Title and Work Package(s): Identification and characterisation of novel antimicrobial plant extracts using stress-sensing biosensors (WP5)
Objectives: To: (1) exploit the stress-sensing biosensors generated by participants UC and NUIG to screen plant extracts and an existing compound library for antimicrobial activity against L. monocytogenes and B. subtilis; (2) extract, purify and chemically characterise novel plant compounds that have antimicrobial activity; (3) characterise the effects of the newly isolated antimicrobial compounds on cell physiology and virulence in collaboration with CSIC; (4) examine the effectiveness of the novel antimicrobials in food trials.
Expected Results: Development of a new stress-sensing biosensor screen for antimicrobial compounds. Isolation of novel antimicrobial plant extracts and characterisation of their effects on bacterial cells. Validation of the new antimicrobial compounds in a food trial.